polyclonal rabbit anti human bmp 2 antibody  (Boster Bio)

Journal: eLife

Article Title: Direct reprogramming of human smooth muscle and vascular endothelial cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome

doi: 10.7554/eLife.54383

Figure Lengend Snippet: From the left: panel one is BMP2, panel two is αSMA, panel three is DAPI and panel four is merge.

Article Snippet: Antibody , Anti-human BMP2 (Rabbit polyclonal) , Novus , Cat#nBP1-19751 RRID: AB_2227877 , IF(1:100).

Techniques:

Journal: eLife

Article Title: Direct reprogramming of human smooth muscle and vascular endothelial cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome

doi: 10.7554/eLife.54383

Figure Lengend Snippet: From the left: panel one is BMP2, panel two is αSMA, panel three is DAPI and panel four is merge.

Article Snippet: Antibody , Anti-human BMP2 (Rabbit polyclonal) , Novus , Cat#nBP1-19751 RRID: AB_2227877 , IF(1:100).

Techniques:

Journal: eLife

Article Title: Direct reprogramming of human smooth muscle and vascular endothelial cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome

doi: 10.7554/eLife.54383

Figure Lengend Snippet: Heat-map representing DE genes between reprogrammed vascular cells from young (N = 3) vs. old (N = 3) donors (iSMCs ( A ), iVECs ( B )). Z score = ± 1.5. DE genes with log 2 FC > 1 and FDR < 0.05. Quantification of BMP2 ( C ) and PALD1 ( D ) expression in human skin biopsies from young vs. old donors (N = 2 donors per condition; N = 10 tissue sections per condition; Student’s t-test with p<0.001 (***) and p<0.05 (*)). Representative images of BMP2 ( E ) and PALD1 ( F ) from skin biopsies obtained from young vs. old donors. Scale bars: 50 µm. Quantification of vascular permeability using young vs. old reprogrammed cells ( G–J ). Schematic showing the generation of an endothelial monolayer covering the interface with a 3D matrix embedding SMCs. Vascular permeability was quantified by measuring the variation of 70 kDa dextran fluorescent intensity across the interface ( G ). Representative images of dextran flow (red) through the endothelial monolayer in presence of young vs. old reprogrammed cells. Scale bar: 100 µm ( H ). Quantification of vascular permeability in presence of young vs. old iVECs (I, at least N = 40 independent measurements per condition in three biological replicates; ANOVA with Holm-Sidak test; comparison with primary VECs (** is p<0.01 and *** is p<0.001); comparison with young iVECs (♦♦♦is p<0.001); comparison with old iVECs (••• is p<0.001)) or young vs. old iSMCs (J, at least N = 40 independent measurements per condition in three biological replicates; ANOVA with Holm-Sidak test; * is p<0.05, ** is p<0.01 and *** is p<0.001). Quantification of vascular permeability in presence of PALD1 KD ( K ) or BMP2 KD ( L ) in primary VECs and SMCs, respectively (at least N = 60 independent measurements per condition in three biological replicates for each KD experiment; Student's t-test; *** is p<0.001).

Article Snippet: Antibody , Anti-human BMP2 (Rabbit polyclonal) , Novus , Cat#nBP1-19751 RRID: AB_2227877 , IF(1:100).

Techniques: Expressing, Permeability, Comparison

Journal: eLife

Article Title: Direct reprogramming of human smooth muscle and vascular endothelial cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome

doi: 10.7554/eLife.54383

Figure Lengend Snippet: Note that BMP2 KD was validated using qPCR due to technical problems with any of the tested anti-BMP2 antibodies (Western Blot). The use of these antibodies resulted in multiple aspecific bands.

Article Snippet: Antibody , Anti-human BMP2 (Rabbit polyclonal) , Novus , Cat#nBP1-19751 RRID: AB_2227877 , IF(1:100).

Techniques: Western Blot

Journal: eLife

Article Title: Direct reprogramming of human smooth muscle and vascular endothelial cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome

doi: 10.7554/eLife.54383

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-human BMP2 (Rabbit polyclonal) , Novus , Cat#nBP1-19751 RRID: AB_2227877 , IF(1:100).

Techniques: Transfection, Construct, Retroviral, Expressing, Isolation, Clinical Proteomics, Blocking Assay, Sequencing, Enzyme-linked Immunosorbent Assay, Software